Journal: Cancer Research Communications

Article Title: Novel Syngeneic Cell Lines for Studying High-Risk BRAF V600E -Driven Colorectal Cancer In Vivo

doi: 10.1158/2767-9764.CRC-25-0599

Figure Lengend Snippet: All NaJa cell lines successfully engraft into syngeneic immunocompetent C57BL/6N mice and recapitulate their individual 2D morphologies in vivo . A, Schematic overview of the PVI of NaJa cells ( n = 2 NaJa-D, n = 10 NaJa-F, and n = 3 NaJa-G). B, Left, livers showing metastatic nodules 4 weeks after injection (black arrows) and invasive growth in the pancreatic tissue (blue circles). Scale bar, 1 cm. Hematoxylin and eosin: Histology showing that all NaJa cell lines establish tumors (pale blue) in the liver parenchyma (pink) with a differentiation state matching their epithelial or mesenchymal in vitro morphology. Right, IHC and immunofluorescence (IF) staining of proliferation marker Ki-67 (left) and intestinal differentiation markers CDX2 (middle) and E-cadherin (right). L, liver; T, tumor. Scale bar (overview), 500 μm, scale bar (zoom, IHC, and IF), 50 μm. C, Quantification of Ki-67 and CDX2 IHC staining in NaJa cell–induced liver metastases. Each dot represents an individual tumor; different shapes indicate individual tissue sections, and colors denote individual mice. Data are shown as the median ±95% confidence interval (CI).Statistical significance was assessed using the Kruskal–Wallis test followed by Dunn multiple comparison test. **, P < 0.01; ****, P < 0.0001. D, IHC staining of ERK and its downstream target DUSP6. Red arrows indicate examples of nuclear ERK staining. L, liver; T, tumor. Scale bar, 50 μm. [ A, Created by J. Traichel in BioRender. Brummer, T. (2025) https://BioRender.com/8ogg64c .]

Article Snippet: The formalin-fixed, paraffin-embedded (FFPE) sections underwent standard processing and staining procedures using the following primary antibodies directed against E-cadherin (1:50, #610181, BD Laboratories; RRID: AB_397580) and Ki-67 (1:400, #9129, Cell Signaling Technology; RRID: AB_2687446).

Techniques: In Vivo, Injection, In Vitro, Immunofluorescence, Staining, Marker, Immunohistochemistry, Comparison

Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet:

Article Snippet: Anti-Ki-67 (B56)-168Er—100 Tests (1:100) , Fluidigm , Cat#3168007B.

Techniques: Recombinant, Saline, Staining, Blocking Assay, Isolation, Software, Cell Counting, Cytometry

Journal: Archives of Gynecology and Obstetrics

Article Title: rHGF interacts with rIGF-1 to activate the satellite cells in the striated urethral sphincter in rats: a promising treatment for urinary incontinence?

doi: 10.1007/s00404-018-4930-2

Figure Lengend Snippet: In Ki-67 staining, all nuclei were stained red with PI. The satellite cells expressing Ki67 were stained blue with Ki67 antibody. In MYH3 and NCAM staining, all nuclei were stained blue with DAPI. The satellite cells were stained red with MYH3 and NCAM antibody. All of the images are merged and show co-localization

Article Snippet: To detect Ki67, after cultivated with BSA for 45 min, the sections were incubated overnight at 4 °C with the Ki67 antibody (CST; 12075S), Propidium bromide (PI) was applied to the sections for 30 min to identify nuclei.

Techniques: Staining, Expressing

Journal: Frontiers in Endocrinology

Article Title: Ultrasound combined with microbubbles promotes diabetic wound healing by regulating macrophage polarization

doi: 10.3389/fendo.2026.1781906

Figure Lengend Snippet: Immunohistochemical staining of wounds in diabetic rats. (A) Representative images of Ki67 immunohistochemical staining in healing tissues from three experimental groups; (B) Quantitative analysis of Ki67-positive cells across groups; (C) Immunohistochemical staining of CD31 in diabetic wounds; (D) Statistical results of CD31-positive expression. Data are presented as mean ± SD (n = 4); *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: After three washes with PBS, they were incubated overnight at 4 °C with anti-CD31 antibodies (1:1000, abcam) and anti-Ki67 antibodies (1:1000, Proteintech).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: European journal of medical research

Article Title: Therapeutic efficacy of 5-alkylresorcinol on progression of colorectal cancer by activating HCLS1 and suppressing TLR4/MYD88/NF-κB signaling.

doi: 10.1186/s40001-025-02775-1

Figure Lengend Snippet: Fig. 6 Effect of 5ARs and overexpression of HCLS1 on tumor cells, genes, and proteins in subcutaneous tumor tissues (n = 3). A Immunohistochemistry detects the proliferation of Ki67 in subcutaneous tumors in nude mice. The brown color indicates positive Ki67 cells (magnification = 400 ×, scale bar = 50 μm). B RT-qPCR detects the effect of 5ARs and HCLS1 on gene expression in tumor tissues. C Western blot detects the effect of 5ARs and HCLS1 protein expression in tumor tissues. N = 3, * Indicates comparison with control. # indicates a comparison with 5ARs. ^ indicates comparison with 5ARs + AAV- blank vector group. */#/^ p < 0.05, **/##/^^ p < 0.01. 5ARs + AAV-blank vector: 5ARs + adeno-associated virus (AAV) empty overexpression vector group. 5ARs + AAV-OE-HCLS1: 5ARs + AAV overexpression vector of HCLS1

Article Snippet: After the addition of goat serum, the sections were incubated with the primary antibody Ki67 Rabbit mAb (A20018, Abclonal, Wuhan, China) (at a dilution ratio of 1:200) following a spin-dry step to remove excess blocking solution.

Techniques: Over Expression, Immunohistochemistry, Quantitative RT-PCR, Gene Expression, Western Blot, Expressing, Comparison, Control, Plasmid Preparation, Virus

Journal: Communications Biology

Article Title: Engineering CCR2/IFN-γ overexpression in enucleated mesenchymal stem cells enhances therapy for rheumatoid arthritis

doi: 10.1038/s42003-025-09248-5

Figure Lengend Snippet: A Schematic diagram of the transwell assay showing the therapeutic effects of En-MSCs C/I and MSCs on damage chondrocytes in vitro. B Fluorescence imaging of Ki67 (red) demonstrating the effects of different treatments on damage chondrocytes in vitro. Scale bar = 200 μm. C Immunofluorescence statistical analysis of Ki67 levels in chondrocytes from different treatments. D qPCR analysis of cartilage development-related genes in different groups. Mean ± s.e.m. ( n = 3 per group, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). E Representative knee morphologies from n = 6 independent CIA rats treated with MSCs or En-MSCs C/I for 6 weeks. Arrows indicating obvious cartilage damage and synovial hyperplasia. Scale bar = 2 mm. F Representative pathological images of cartilages treated with En-MSCs C/I or MSCs. Joints were stained with safranin O, toluidine blue, and immunohistochemical staining for ACAN and COL2. Scale bar = 200 μm. G Histological scores based on analysis of synovial hyperplasia, inflammatory cell infiltration, bone destruction, and cartilage destruction. Mean ± s.e.m. ( n = 6 per group, * p < 0.05, ** p < 0.01, **** p < 0.0001). H COL2+ and ACAN+ chondrocytes were quantified. Mean ± s.e.m. ( n = 6 per group, **** p < 0.0001). All error bars denote Mean ± s.e.m.; dots indicate individual sam p les/rats.

Article Snippet: For immunofluorescence staining, the following primary antibodies were used: CD74(SantaCruz,sc-6262,1:100), Prg4(ABCAM,28484,1:400),TREM2 antibody (invitrogen, 3AABDA30, 1:150), CD68 antibody (invitrogen,2740420, 1:200), CXCL12 antibody (Santa Cruz,sc-74271, 1:50), HBEGF antibody (Santa Cruz,sc-365182, 1:150), CLEC10A antibody (Santa Cruz,sc-365182, 1:50), Ly6c antibody (ABCAM,ab318272, 1:100), CD68 antibody (ABCAM,ab283654, 1:200), CD34 antibody (ABCAM,ab81289, 1:50), COL2 antibody (ABCAM,ab34712, 1:300) and Ki67 (Abclonal,A16919, 1:200).

Techniques: Transwell Assay, In Vitro, Fluorescence, Imaging, Immunofluorescence, Staining, Immunohistochemical staining